This Draft Rwanda Standard provides requirements for the design, construction, operation, maintenance, management, and biosafety practices for plant biotechnology facilities operating at Biosafety Level 2 (BSL-2). It applies to all research and development activities that may pose moderate risk to human and environment. It covers laboratories, growth rooms, greenhouses, containment rooms, and associated support areas used for plant biotechnology activities involving biological agents and materials that require BSL-2 containment. Specifically, it focuses on: a) facility design and containment requirements; b) biosafety and biosecurity management; c) personnel competence and training; d) operational and work practices; e) equipment requirements; f) waste management and decontamination; g) emergency preparedness and response; and h) environmental and occupational safety measures. It does not apply to: — Biosafety Level 1 (BSL-1), Biosafety Level 3 (BSL-3), or Biosafety Level 4 (BSL-4) facilities; — open field trials or environmental release activities; or — facilities intended exclusively for medical or animal diagnostic activities.