This draft Notification of the Ministry of Public Health (No....) B.E..... was issued by virtue of the Food Act, B.E. 2522 (1979), regarding Enzymes Used in Food Production (No. 2). Its objective is to update the list of enzymes that have passed safety assessments by the Thai FDA and JECFA. This version repeals and replaces the previous enzyme lists in Schedule 1 of the Notification of the Ministry of Public Health (No. 443) B.E. 2566 (2023), issued under the Food Act, B.E. 2522 (1979), regarding Enzymes Used in Food Production. Furthermore, the draft includes Silicon dioxide (INS 551) in the list of permitted food additives for enzyme preparations under Schedule 4.